anti b actin Search Results


rabbit anti β actin  (Rockland Immunochemicals)
91
Rockland Immunochemicals rabbit anti β actin
MEIS2 is essential for neuroblastoma cell survival. ( a ) Immunoblotting of MEIS2 in a panel of 13 human neuroblastoma cell lines. β -Actin levels are shown as a loading control. ( b and c ) qRT-PCR ( b ) and immunoblot ( c ) analyses of MEIS2 expression in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. Error bars ( b ) represent S.D. ( n =3). GAPDH ( c ) serves as a loading control. ( d ) Micrographs of BE(2)-C cells infected for 3 days with lentiviruses expressing either shGFP or shMEIS2. ( e ) Trypan blue exclusion assay of viable BE(2)-C cells infected for 4 days with lentiviruses expressing either shGFP or shMEIS2
Rabbit Anti β Actin, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti β actin/product/Rockland Immunochemicals
Average 91 stars, based on 1 article reviews
rabbit anti β actin - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier
β-actin antibody  (Oncogene Science Inc)
90
Oncogene Science Inc β-actin antibody
MEIS2 is essential for neuroblastoma cell survival. ( a ) Immunoblotting of MEIS2 in a panel of 13 human neuroblastoma cell lines. β -Actin levels are shown as a loading control. ( b and c ) qRT-PCR ( b ) and immunoblot ( c ) analyses of MEIS2 expression in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. Error bars ( b ) represent S.D. ( n =3). GAPDH ( c ) serves as a loading control. ( d ) Micrographs of BE(2)-C cells infected for 3 days with lentiviruses expressing either shGFP or shMEIS2. ( e ) Trypan blue exclusion assay of viable BE(2)-C cells infected for 4 days with lentiviruses expressing either shGFP or shMEIS2
β Actin Antibody, supplied by Oncogene Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β-actin antibody/product/Oncogene Science Inc
Average 90 stars, based on 1 article reviews
β-actin antibody - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
antibody against the internal control protein β-actin  (Beyotime)
90
Beyotime antibody against the internal control protein β-actin
MEIS2 is essential for neuroblastoma cell survival. ( a ) Immunoblotting of MEIS2 in a panel of 13 human neuroblastoma cell lines. β -Actin levels are shown as a loading control. ( b and c ) qRT-PCR ( b ) and immunoblot ( c ) analyses of MEIS2 expression in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. Error bars ( b ) represent S.D. ( n =3). GAPDH ( c ) serves as a loading control. ( d ) Micrographs of BE(2)-C cells infected for 3 days with lentiviruses expressing either shGFP or shMEIS2. ( e ) Trypan blue exclusion assay of viable BE(2)-C cells infected for 4 days with lentiviruses expressing either shGFP or shMEIS2
Antibody Against The Internal Control Protein β Actin, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody against the internal control protein β-actin/product/Beyotime
Average 90 stars, based on 1 article reviews
antibody against the internal control protein β-actin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti-b-actin  (Verlag GmbH)
90
Verlag GmbH anti-b-actin
MEIS2 is essential for neuroblastoma cell survival. ( a ) Immunoblotting of MEIS2 in a panel of 13 human neuroblastoma cell lines. β -Actin levels are shown as a loading control. ( b and c ) qRT-PCR ( b ) and immunoblot ( c ) analyses of MEIS2 expression in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. Error bars ( b ) represent S.D. ( n =3). GAPDH ( c ) serves as a loading control. ( d ) Micrographs of BE(2)-C cells infected for 3 days with lentiviruses expressing either shGFP or shMEIS2. ( e ) Trypan blue exclusion assay of viable BE(2)-C cells infected for 4 days with lentiviruses expressing either shGFP or shMEIS2
Anti B Actin, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-b-actin/product/Verlag GmbH
Average 90 stars, based on 1 article reviews
anti-b-actin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti-b-actin igg  (Merck & Co)
90
Merck & Co anti-b-actin igg
MEIS2 is essential for neuroblastoma cell survival. ( a ) Immunoblotting of MEIS2 in a panel of 13 human neuroblastoma cell lines. β -Actin levels are shown as a loading control. ( b and c ) qRT-PCR ( b ) and immunoblot ( c ) analyses of MEIS2 expression in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. Error bars ( b ) represent S.D. ( n =3). GAPDH ( c ) serves as a loading control. ( d ) Micrographs of BE(2)-C cells infected for 3 days with lentiviruses expressing either shGFP or shMEIS2. ( e ) Trypan blue exclusion assay of viable BE(2)-C cells infected for 4 days with lentiviruses expressing either shGFP or shMEIS2
Anti B Actin Igg, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-b-actin igg/product/Merck & Co
Average 90 stars, based on 1 article reviews
anti-b-actin igg - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
rabbit polyclonal antibodies to β-actin  (Sangon Biotech)
90
Sangon Biotech rabbit polyclonal antibodies to β-actin
Effect of ALAPP on the levels of HSF1, HSP90, CDK4, and AKT1 expression by MG-63 osteosarcoma cells and level of Hsf1, Hsp90, Cdk4, and Akt1 expression by MC3T3 osteoblasts. The levels of protein expression were determined by western blotting. After treatment with 50 μg/mL ALAPP for 48 h, MC3T3 osteoblasts and MG-63 osteosarcoma cells were collected and mixed with 100 μL xTractor Buffer (Clontech). Total cell protein extracts were adjusted to the same protein concentration using the Bradford method, and their protein components were separated by electrophoresis in a 12% polyacrylamide gel containing SDS and transferred to PVDF membranes. Primary antibodies, each with immunoreactivity for human and mouse homologs of the proteins, were purchased from Sangon Biotech, including the rabbit polyclonal antibodies <t>anti-ACTC1</t> (catalog No. <t>D224905),</t> anti-HSF1 (catalog No. D220782), anti-HSP90AA1 (catalog No. D220009), anti-CDK4 (catalog No. D120396), and anti-AKT1(Ab-129) (catalog No. D151616). Immunoreactivity was detected with secondary antibody HRP-conjugated goat anti-rabbit IgG (catalog No. D110058, Sangon) using an ECL chemiluminescence substrate (catalog No. T7101A, TaKaRa). Expression of the various proteins was calculated relative to <t>β-actin</t> used as a control. Each bar represents the average of n = 3 and error bars indicate standard deviation. Student t tests were conducted to determine differences in expression. * P < 0.05, ** P < 0.005 vs untreated control cells. A . HSF1. B. HSP90. C. CDK4, D. AKT1. ACTC1, β-actin; AKT1, serine-threonine protein kinase encoded by AKT1 ; ALAPP, antlion antiproliferative polypeptide; CDK4, cyclin-dependent kinase 4; HRP, horseradish peroxidase; HSF1, heat shock transcription factor 1; HSP90, heat shock protein 90 kDa alpha (cytosolic), class A member 1 (HSP90AA1); PVDF, polyvinylidene difluoride.
Rabbit Polyclonal Antibodies To β Actin, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibodies to β-actin/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibodies to β-actin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
b -actin probe  (Boehringer Mannheim)
90
Boehringer Mannheim b -actin probe
Effect of ALAPP on the levels of HSF1, HSP90, CDK4, and AKT1 expression by MG-63 osteosarcoma cells and level of Hsf1, Hsp90, Cdk4, and Akt1 expression by MC3T3 osteoblasts. The levels of protein expression were determined by western blotting. After treatment with 50 μg/mL ALAPP for 48 h, MC3T3 osteoblasts and MG-63 osteosarcoma cells were collected and mixed with 100 μL xTractor Buffer (Clontech). Total cell protein extracts were adjusted to the same protein concentration using the Bradford method, and their protein components were separated by electrophoresis in a 12% polyacrylamide gel containing SDS and transferred to PVDF membranes. Primary antibodies, each with immunoreactivity for human and mouse homologs of the proteins, were purchased from Sangon Biotech, including the rabbit polyclonal antibodies <t>anti-ACTC1</t> (catalog No. <t>D224905),</t> anti-HSF1 (catalog No. D220782), anti-HSP90AA1 (catalog No. D220009), anti-CDK4 (catalog No. D120396), and anti-AKT1(Ab-129) (catalog No. D151616). Immunoreactivity was detected with secondary antibody HRP-conjugated goat anti-rabbit IgG (catalog No. D110058, Sangon) using an ECL chemiluminescence substrate (catalog No. T7101A, TaKaRa). Expression of the various proteins was calculated relative to <t>β-actin</t> used as a control. Each bar represents the average of n = 3 and error bars indicate standard deviation. Student t tests were conducted to determine differences in expression. * P < 0.05, ** P < 0.005 vs untreated control cells. A . HSF1. B. HSP90. C. CDK4, D. AKT1. ACTC1, β-actin; AKT1, serine-threonine protein kinase encoded by AKT1 ; ALAPP, antlion antiproliferative polypeptide; CDK4, cyclin-dependent kinase 4; HRP, horseradish peroxidase; HSF1, heat shock transcription factor 1; HSP90, heat shock protein 90 kDa alpha (cytosolic), class A member 1 (HSP90AA1); PVDF, polyvinylidene difluoride.
B Actin Probe, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/b -actin probe/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
b -actin probe - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
mouse anti-b-actin, mab  (Progen Biotechnik)
90
Progen Biotechnik mouse anti-b-actin, mab
Effect of ALAPP on the levels of HSF1, HSP90, CDK4, and AKT1 expression by MG-63 osteosarcoma cells and level of Hsf1, Hsp90, Cdk4, and Akt1 expression by MC3T3 osteoblasts. The levels of protein expression were determined by western blotting. After treatment with 50 μg/mL ALAPP for 48 h, MC3T3 osteoblasts and MG-63 osteosarcoma cells were collected and mixed with 100 μL xTractor Buffer (Clontech). Total cell protein extracts were adjusted to the same protein concentration using the Bradford method, and their protein components were separated by electrophoresis in a 12% polyacrylamide gel containing SDS and transferred to PVDF membranes. Primary antibodies, each with immunoreactivity for human and mouse homologs of the proteins, were purchased from Sangon Biotech, including the rabbit polyclonal antibodies <t>anti-ACTC1</t> (catalog No. <t>D224905),</t> anti-HSF1 (catalog No. D220782), anti-HSP90AA1 (catalog No. D220009), anti-CDK4 (catalog No. D120396), and anti-AKT1(Ab-129) (catalog No. D151616). Immunoreactivity was detected with secondary antibody HRP-conjugated goat anti-rabbit IgG (catalog No. D110058, Sangon) using an ECL chemiluminescence substrate (catalog No. T7101A, TaKaRa). Expression of the various proteins was calculated relative to <t>β-actin</t> used as a control. Each bar represents the average of n = 3 and error bars indicate standard deviation. Student t tests were conducted to determine differences in expression. * P < 0.05, ** P < 0.005 vs untreated control cells. A . HSF1. B. HSP90. C. CDK4, D. AKT1. ACTC1, β-actin; AKT1, serine-threonine protein kinase encoded by AKT1 ; ALAPP, antlion antiproliferative polypeptide; CDK4, cyclin-dependent kinase 4; HRP, horseradish peroxidase; HSF1, heat shock transcription factor 1; HSP90, heat shock protein 90 kDa alpha (cytosolic), class A member 1 (HSP90AA1); PVDF, polyvinylidene difluoride.
Mouse Anti B Actin, Mab, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-b-actin, mab/product/Progen Biotechnik
Average 90 stars, based on 1 article reviews
mouse anti-b-actin, mab - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
antib actin  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc antib actin
Effect of ALAPP on the levels of HSF1, HSP90, CDK4, and AKT1 expression by MG-63 osteosarcoma cells and level of Hsf1, Hsp90, Cdk4, and Akt1 expression by MC3T3 osteoblasts. The levels of protein expression were determined by western blotting. After treatment with 50 μg/mL ALAPP for 48 h, MC3T3 osteoblasts and MG-63 osteosarcoma cells were collected and mixed with 100 μL xTractor Buffer (Clontech). Total cell protein extracts were adjusted to the same protein concentration using the Bradford method, and their protein components were separated by electrophoresis in a 12% polyacrylamide gel containing SDS and transferred to PVDF membranes. Primary antibodies, each with immunoreactivity for human and mouse homologs of the proteins, were purchased from Sangon Biotech, including the rabbit polyclonal antibodies <t>anti-ACTC1</t> (catalog No. <t>D224905),</t> anti-HSF1 (catalog No. D220782), anti-HSP90AA1 (catalog No. D220009), anti-CDK4 (catalog No. D120396), and anti-AKT1(Ab-129) (catalog No. D151616). Immunoreactivity was detected with secondary antibody HRP-conjugated goat anti-rabbit IgG (catalog No. D110058, Sangon) using an ECL chemiluminescence substrate (catalog No. T7101A, TaKaRa). Expression of the various proteins was calculated relative to <t>β-actin</t> used as a control. Each bar represents the average of n = 3 and error bars indicate standard deviation. Student t tests were conducted to determine differences in expression. * P < 0.05, ** P < 0.005 vs untreated control cells. A . HSF1. B. HSP90. C. CDK4, D. AKT1. ACTC1, β-actin; AKT1, serine-threonine protein kinase encoded by AKT1 ; ALAPP, antlion antiproliferative polypeptide; CDK4, cyclin-dependent kinase 4; HRP, horseradish peroxidase; HSF1, heat shock transcription factor 1; HSP90, heat shock protein 90 kDa alpha (cytosolic), class A member 1 (HSP90AA1); PVDF, polyvinylidene difluoride.
Antib Actin, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antib actin/product/Amersham Life Sciences Inc
Average 90 stars, based on 1 article reviews
antib actin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
monoclonal antibody against human β-actin  (Alpha Diagnostics)
90
Alpha Diagnostics monoclonal antibody against human β-actin
Effect of ALAPP on the levels of HSF1, HSP90, CDK4, and AKT1 expression by MG-63 osteosarcoma cells and level of Hsf1, Hsp90, Cdk4, and Akt1 expression by MC3T3 osteoblasts. The levels of protein expression were determined by western blotting. After treatment with 50 μg/mL ALAPP for 48 h, MC3T3 osteoblasts and MG-63 osteosarcoma cells were collected and mixed with 100 μL xTractor Buffer (Clontech). Total cell protein extracts were adjusted to the same protein concentration using the Bradford method, and their protein components were separated by electrophoresis in a 12% polyacrylamide gel containing SDS and transferred to PVDF membranes. Primary antibodies, each with immunoreactivity for human and mouse homologs of the proteins, were purchased from Sangon Biotech, including the rabbit polyclonal antibodies <t>anti-ACTC1</t> (catalog No. <t>D224905),</t> anti-HSF1 (catalog No. D220782), anti-HSP90AA1 (catalog No. D220009), anti-CDK4 (catalog No. D120396), and anti-AKT1(Ab-129) (catalog No. D151616). Immunoreactivity was detected with secondary antibody HRP-conjugated goat anti-rabbit IgG (catalog No. D110058, Sangon) using an ECL chemiluminescence substrate (catalog No. T7101A, TaKaRa). Expression of the various proteins was calculated relative to <t>β-actin</t> used as a control. Each bar represents the average of n = 3 and error bars indicate standard deviation. Student t tests were conducted to determine differences in expression. * P < 0.05, ** P < 0.005 vs untreated control cells. A . HSF1. B. HSP90. C. CDK4, D. AKT1. ACTC1, β-actin; AKT1, serine-threonine protein kinase encoded by AKT1 ; ALAPP, antlion antiproliferative polypeptide; CDK4, cyclin-dependent kinase 4; HRP, horseradish peroxidase; HSF1, heat shock transcription factor 1; HSP90, heat shock protein 90 kDa alpha (cytosolic), class A member 1 (HSP90AA1); PVDF, polyvinylidene difluoride.
Monoclonal Antibody Against Human β Actin, supplied by Alpha Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal antibody against human β-actin/product/Alpha Diagnostics
Average 90 stars, based on 1 article reviews
monoclonal antibody against human β-actin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
primary antibodies bcl-2, bax, β-actin  (Wanleibio)
90
Wanleibio primary antibodies bcl-2, bax, β-actin
Effect of ALAPP on the levels of HSF1, HSP90, CDK4, and AKT1 expression by MG-63 osteosarcoma cells and level of Hsf1, Hsp90, Cdk4, and Akt1 expression by MC3T3 osteoblasts. The levels of protein expression were determined by western blotting. After treatment with 50 μg/mL ALAPP for 48 h, MC3T3 osteoblasts and MG-63 osteosarcoma cells were collected and mixed with 100 μL xTractor Buffer (Clontech). Total cell protein extracts were adjusted to the same protein concentration using the Bradford method, and their protein components were separated by electrophoresis in a 12% polyacrylamide gel containing SDS and transferred to PVDF membranes. Primary antibodies, each with immunoreactivity for human and mouse homologs of the proteins, were purchased from Sangon Biotech, including the rabbit polyclonal antibodies <t>anti-ACTC1</t> (catalog No. <t>D224905),</t> anti-HSF1 (catalog No. D220782), anti-HSP90AA1 (catalog No. D220009), anti-CDK4 (catalog No. D120396), and anti-AKT1(Ab-129) (catalog No. D151616). Immunoreactivity was detected with secondary antibody HRP-conjugated goat anti-rabbit IgG (catalog No. D110058, Sangon) using an ECL chemiluminescence substrate (catalog No. T7101A, TaKaRa). Expression of the various proteins was calculated relative to <t>β-actin</t> used as a control. Each bar represents the average of n = 3 and error bars indicate standard deviation. Student t tests were conducted to determine differences in expression. * P < 0.05, ** P < 0.005 vs untreated control cells. A . HSF1. B. HSP90. C. CDK4, D. AKT1. ACTC1, β-actin; AKT1, serine-threonine protein kinase encoded by AKT1 ; ALAPP, antlion antiproliferative polypeptide; CDK4, cyclin-dependent kinase 4; HRP, horseradish peroxidase; HSF1, heat shock transcription factor 1; HSP90, heat shock protein 90 kDa alpha (cytosolic), class A member 1 (HSP90AA1); PVDF, polyvinylidene difluoride.
Primary Antibodies Bcl 2, Bax, β Actin, supplied by Wanleibio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies bcl-2, bax, β-actin/product/Wanleibio
Average 90 stars, based on 1 article reviews
primary antibodies bcl-2, bax, β-actin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti-b-actin  (Seikagaku corporation)
90
Seikagaku corporation anti-b-actin
Effect of ALAPP on the levels of HSF1, HSP90, CDK4, and AKT1 expression by MG-63 osteosarcoma cells and level of Hsf1, Hsp90, Cdk4, and Akt1 expression by MC3T3 osteoblasts. The levels of protein expression were determined by western blotting. After treatment with 50 μg/mL ALAPP for 48 h, MC3T3 osteoblasts and MG-63 osteosarcoma cells were collected and mixed with 100 μL xTractor Buffer (Clontech). Total cell protein extracts were adjusted to the same protein concentration using the Bradford method, and their protein components were separated by electrophoresis in a 12% polyacrylamide gel containing SDS and transferred to PVDF membranes. Primary antibodies, each with immunoreactivity for human and mouse homologs of the proteins, were purchased from Sangon Biotech, including the rabbit polyclonal antibodies <t>anti-ACTC1</t> (catalog No. <t>D224905),</t> anti-HSF1 (catalog No. D220782), anti-HSP90AA1 (catalog No. D220009), anti-CDK4 (catalog No. D120396), and anti-AKT1(Ab-129) (catalog No. D151616). Immunoreactivity was detected with secondary antibody HRP-conjugated goat anti-rabbit IgG (catalog No. D110058, Sangon) using an ECL chemiluminescence substrate (catalog No. T7101A, TaKaRa). Expression of the various proteins was calculated relative to <t>β-actin</t> used as a control. Each bar represents the average of n = 3 and error bars indicate standard deviation. Student t tests were conducted to determine differences in expression. * P < 0.05, ** P < 0.005 vs untreated control cells. A . HSF1. B. HSP90. C. CDK4, D. AKT1. ACTC1, β-actin; AKT1, serine-threonine protein kinase encoded by AKT1 ; ALAPP, antlion antiproliferative polypeptide; CDK4, cyclin-dependent kinase 4; HRP, horseradish peroxidase; HSF1, heat shock transcription factor 1; HSP90, heat shock protein 90 kDa alpha (cytosolic), class A member 1 (HSP90AA1); PVDF, polyvinylidene difluoride.
Anti B Actin, supplied by Seikagaku corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-b-actin/product/Seikagaku corporation
Average 90 stars, based on 1 article reviews
anti-b-actin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


MEIS2 is essential for neuroblastoma cell survival. ( a ) Immunoblotting of MEIS2 in a panel of 13 human neuroblastoma cell lines. β -Actin levels are shown as a loading control. ( b and c ) qRT-PCR ( b ) and immunoblot ( c ) analyses of MEIS2 expression in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. Error bars ( b ) represent S.D. ( n =3). GAPDH ( c ) serves as a loading control. ( d ) Micrographs of BE(2)-C cells infected for 3 days with lentiviruses expressing either shGFP or shMEIS2. ( e ) Trypan blue exclusion assay of viable BE(2)-C cells infected for 4 days with lentiviruses expressing either shGFP or shMEIS2

Journal: Cell Death & Disease

Article Title: MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression

doi: 10.1038/cddis.2014.370

Figure Lengend Snippet: MEIS2 is essential for neuroblastoma cell survival. ( a ) Immunoblotting of MEIS2 in a panel of 13 human neuroblastoma cell lines. β -Actin levels are shown as a loading control. ( b and c ) qRT-PCR ( b ) and immunoblot ( c ) analyses of MEIS2 expression in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. Error bars ( b ) represent S.D. ( n =3). GAPDH ( c ) serves as a loading control. ( d ) Micrographs of BE(2)-C cells infected for 3 days with lentiviruses expressing either shGFP or shMEIS2. ( e ) Trypan blue exclusion assay of viable BE(2)-C cells infected for 4 days with lentiviruses expressing either shGFP or shMEIS2

Article Snippet: Immunoblotting was conducted according to standard procedures using the following primary antibodies: rabbit anti-BCL2 (sc-783, 1 : 200), rabbit anti-caspase 3 (sc-7148, 1 : 200), rabbit anti-Flag (F7425, 1 : 1000; Sigma-Aldrich), rabbit anti-FOXM1 (sc-502, 1 : 100), rabbit anti-GAPDH (sc-25778, 1 : 3000), mouse anti-MEIS2 (sc-81986, 1 : 400), rabbit anti- β -actin (600-401-886, 1 : 2000; Rockland Immunochemicals, Gilbertsville, PA, USA), and mouse anti- α -tubulin (B-5-1-2, 1 : 5000; Sigma-Aldrich).

Techniques: Western Blot, Quantitative RT-PCR, Expressing, Infection, Trypan Blue Exclusion Assay

MEIS2 targets the MuvB-BMYB-FOXM1 complex for transcriptional control of M-phase progression. ( a ) qRT-PCR analysis of mRNA expression of MEIS2, FOXM1, BMYB, and RBBP4 in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. ( b ) Immunoblotting of MEIS2 and FOXM1 in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. β -Actin levels are shown as a loading control. MEIS2 and FOXM1 levels were quantified against β -actin. ( c ) GSEA showing marked downregulation of the FOXM1 pathway genes in BE(2)-C cells with MEIS2 depletion. ( d ) ChIP-qPCR analysis showing MEIS2 binding to the FOXM1 promoter region (−312 to −158) containing a consensus TGIF/MEIS2-binding sequence TGTCA. Error bars, S.D. ( n =3). ( e ) Immunoblotting of FOXM1 in BE(2)-C and SK-N-DZ cells with or without FOXM1 knockdown. β -Actin levels are shown as a loading control. ( f ) qRT-PCR analysis of mRNA expression of key FOXM1 target genes in BE(2)-C and SK-N-DZ cells with or without FOXM1 knockdown. ( g ) Growth assay of BE(2)-C and SK-N-DZ cells with or without FOXM1 knockdown. Error bars, S.D. ( n =3). ** P <0.001. Data ( d and g ) were analyzed with two-tailed Student's t -test

Journal: Cell Death & Disease

Article Title: MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression

doi: 10.1038/cddis.2014.370

Figure Lengend Snippet: MEIS2 targets the MuvB-BMYB-FOXM1 complex for transcriptional control of M-phase progression. ( a ) qRT-PCR analysis of mRNA expression of MEIS2, FOXM1, BMYB, and RBBP4 in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. ( b ) Immunoblotting of MEIS2 and FOXM1 in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. β -Actin levels are shown as a loading control. MEIS2 and FOXM1 levels were quantified against β -actin. ( c ) GSEA showing marked downregulation of the FOXM1 pathway genes in BE(2)-C cells with MEIS2 depletion. ( d ) ChIP-qPCR analysis showing MEIS2 binding to the FOXM1 promoter region (−312 to −158) containing a consensus TGIF/MEIS2-binding sequence TGTCA. Error bars, S.D. ( n =3). ( e ) Immunoblotting of FOXM1 in BE(2)-C and SK-N-DZ cells with or without FOXM1 knockdown. β -Actin levels are shown as a loading control. ( f ) qRT-PCR analysis of mRNA expression of key FOXM1 target genes in BE(2)-C and SK-N-DZ cells with or without FOXM1 knockdown. ( g ) Growth assay of BE(2)-C and SK-N-DZ cells with or without FOXM1 knockdown. Error bars, S.D. ( n =3). ** P <0.001. Data ( d and g ) were analyzed with two-tailed Student's t -test

Article Snippet: Immunoblotting was conducted according to standard procedures using the following primary antibodies: rabbit anti-BCL2 (sc-783, 1 : 200), rabbit anti-caspase 3 (sc-7148, 1 : 200), rabbit anti-Flag (F7425, 1 : 1000; Sigma-Aldrich), rabbit anti-FOXM1 (sc-502, 1 : 100), rabbit anti-GAPDH (sc-25778, 1 : 3000), mouse anti-MEIS2 (sc-81986, 1 : 400), rabbit anti- β -actin (600-401-886, 1 : 2000; Rockland Immunochemicals, Gilbertsville, PA, USA), and mouse anti- α -tubulin (B-5-1-2, 1 : 5000; Sigma-Aldrich).

Techniques: Quantitative RT-PCR, Expressing, Infection, Western Blot, Binding Assay, Sequencing, Growth Assay, Two Tailed Test

Effect of ALAPP on the levels of HSF1, HSP90, CDK4, and AKT1 expression by MG-63 osteosarcoma cells and level of Hsf1, Hsp90, Cdk4, and Akt1 expression by MC3T3 osteoblasts. The levels of protein expression were determined by western blotting. After treatment with 50 μg/mL ALAPP for 48 h, MC3T3 osteoblasts and MG-63 osteosarcoma cells were collected and mixed with 100 μL xTractor Buffer (Clontech). Total cell protein extracts were adjusted to the same protein concentration using the Bradford method, and their protein components were separated by electrophoresis in a 12% polyacrylamide gel containing SDS and transferred to PVDF membranes. Primary antibodies, each with immunoreactivity for human and mouse homologs of the proteins, were purchased from Sangon Biotech, including the rabbit polyclonal antibodies anti-ACTC1 (catalog No. D224905), anti-HSF1 (catalog No. D220782), anti-HSP90AA1 (catalog No. D220009), anti-CDK4 (catalog No. D120396), and anti-AKT1(Ab-129) (catalog No. D151616). Immunoreactivity was detected with secondary antibody HRP-conjugated goat anti-rabbit IgG (catalog No. D110058, Sangon) using an ECL chemiluminescence substrate (catalog No. T7101A, TaKaRa). Expression of the various proteins was calculated relative to β-actin used as a control. Each bar represents the average of n = 3 and error bars indicate standard deviation. Student t tests were conducted to determine differences in expression. * P < 0.05, ** P < 0.005 vs untreated control cells. A . HSF1. B. HSP90. C. CDK4, D. AKT1. ACTC1, β-actin; AKT1, serine-threonine protein kinase encoded by AKT1 ; ALAPP, antlion antiproliferative polypeptide; CDK4, cyclin-dependent kinase 4; HRP, horseradish peroxidase; HSF1, heat shock transcription factor 1; HSP90, heat shock protein 90 kDa alpha (cytosolic), class A member 1 (HSP90AA1); PVDF, polyvinylidene difluoride.

Journal: Asian Biomedicine: Research, Reviews and News

Article Title: An 85-amino-acid polypeptide from Myrmeleon bore larvae (antlions) homologous to heat shock factor binding protein 1 with antiproliferative activity against MG-63 osteosarcoma cells in vitro

doi: 10.2478/abm-2022-0024

Figure Lengend Snippet: Effect of ALAPP on the levels of HSF1, HSP90, CDK4, and AKT1 expression by MG-63 osteosarcoma cells and level of Hsf1, Hsp90, Cdk4, and Akt1 expression by MC3T3 osteoblasts. The levels of protein expression were determined by western blotting. After treatment with 50 μg/mL ALAPP for 48 h, MC3T3 osteoblasts and MG-63 osteosarcoma cells were collected and mixed with 100 μL xTractor Buffer (Clontech). Total cell protein extracts were adjusted to the same protein concentration using the Bradford method, and their protein components were separated by electrophoresis in a 12% polyacrylamide gel containing SDS and transferred to PVDF membranes. Primary antibodies, each with immunoreactivity for human and mouse homologs of the proteins, were purchased from Sangon Biotech, including the rabbit polyclonal antibodies anti-ACTC1 (catalog No. D224905), anti-HSF1 (catalog No. D220782), anti-HSP90AA1 (catalog No. D220009), anti-CDK4 (catalog No. D120396), and anti-AKT1(Ab-129) (catalog No. D151616). Immunoreactivity was detected with secondary antibody HRP-conjugated goat anti-rabbit IgG (catalog No. D110058, Sangon) using an ECL chemiluminescence substrate (catalog No. T7101A, TaKaRa). Expression of the various proteins was calculated relative to β-actin used as a control. Each bar represents the average of n = 3 and error bars indicate standard deviation. Student t tests were conducted to determine differences in expression. * P < 0.05, ** P < 0.005 vs untreated control cells. A . HSF1. B. HSP90. C. CDK4, D. AKT1. ACTC1, β-actin; AKT1, serine-threonine protein kinase encoded by AKT1 ; ALAPP, antlion antiproliferative polypeptide; CDK4, cyclin-dependent kinase 4; HRP, horseradish peroxidase; HSF1, heat shock transcription factor 1; HSP90, heat shock protein 90 kDa alpha (cytosolic), class A member 1 (HSP90AA1); PVDF, polyvinylidene difluoride.

Article Snippet: Primary antibodies, including rabbit polyclonal antibodies to β-actin (anti-ACTC1, catalog No. D224905), heat shock transcription factor 1 (anti-HSF1, catalog No. D220782), heat shock protein 90 kDa alpha (cytosolic), class A member 1 (anti-HSP90AA1, catalog No. D220009), cyclin-dependent kinase 4 (anti-CDK4, catalog No. D120396), and serine-threonine protein kinase encoded by AKT1 (anti-AKT1(Ab-129), catalog No. D151616), each with immunoreactivity for human and mouse homologs of the proteins, and secondary antibody of horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (catalog No. D110058), were purchased from Sangon Biotech.

Techniques: Expressing, Western Blot, Protein Concentration, Electrophoresis, Control, Standard Deviation